Lumafluor紅色Red Retrobeads登2016年Nature應(yīng)用

品牌：Lumafluor

貨號(hào)：R180

名稱：Red Retrobeads™ (100 µl)

國(guó)內(nèi)渠道現(xiàn)貨商：靶點(diǎn)科技

論文題目：Thirst neurons anticipate the homeostatic consequences of eating and drinking

期刊：Nature volume 537, pages 680–684 (2016)

中文摘要：口渴會(huì)促使動(dòng)物喝水以保持體液平衡。口渴通常被視為對(duì)血容量或張力變化的穩(wěn)態(tài)反應(yīng)。然而，大多數(shù)飲酒行為的調(diào)節(jié)速度太快，無(wú)法直接由血液成分控制，相反，似乎在穩(wěn)態(tài)失衡出現(xiàn)之前就預(yù)測(cè)了它們。如何實(shí)現(xiàn)這一點(diǎn)仍然未知。在這里，我們揭示了穹窿下器官（SFO）在小鼠口渴的預(yù)期調(diào)節(jié)中的意想不到的作用。通過(guò)監(jiān)測(cè)深部腦鈣動(dòng)力學(xué)，我們發(fā)現(xiàn)促進(jìn)口渴的SFO神經(jīng)元在進(jìn)食和飲水過(guò)程中對(duì)口腔的輸入做出反應(yīng)，然后將這些輸入與有關(guān)血液成分的信息整合在一起。這種整合使SFO神經(jīng)元能夠預(yù)測(cè)持續(xù)的食物和水消耗將如何改變未來(lái)的體液平衡，然后先發(fā)制人地調(diào)整行為。互補(bǔ)的光遺傳學(xué)操作表明，這種預(yù)期調(diào)節(jié)對(duì)于在幾種情況下的飲酒是必要的。這些發(fā)現(xiàn)提供了一種神經(jīng)機(jī)制來(lái)解釋長(zhǎng)期的行為觀察，包括進(jìn)餐時(shí)飲酒的普遍性，口渴的快速飽腹感，以及口腔冷卻是解渴的事實(shí)。

英文摘要：Thirst motivates animals to drink in order to maintain fluid balance. Thirst has conventionally been viewed as a homeostatic response to changes in blood volume or tonicity. However, most drinking behaviour is regulated too rapidly to be controlled by blood composition directly, and instead seems to anticipate homeostatic imbalances before they arise. How this is achieved remains unknown. Here we reveal an unexpected role for the subfornical organ (SFO) in the anticipatory regulation of thirst in mice. By monitoring deep-brain calcium dynamics, we show that thirst-promoting SFO neurons respond to inputs from the oral cavity during eating and drinking and then integrate these inputs with information about the composition of the blood. This integration allows SFO neurons to predict how ongoing food and water consumption will alter fluid balance in the future and then to adjust behaviour pre-emptively. Complementary optogenetic manipulations show that this anticipatory modulation is necessary for drinking in several contexts. These findings provide a neural mechanism to explain longstanding behavioural observations, including the prevalence of drinking during meals, the rapid satiation of thirst, and the fact that oral cooling is thirst-quenching.

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